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NIR Discussion Forum » Bruce Campbell's List » I need help » Help needed on determination of a-helix, b-sheet and b-turn of peptide in NIR « Previous Next »

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Heather Jones
Posted on Sunday, July 10, 2005 - 12:00 pm:   

Hi, follows,

I just start a new project to study the stuucture of fibrils of peptides with NIR. Traditionaly, It can be monitored by FTIR. For example, 1655 cm-1 for a-helix, 1630 cm-1 for b-sheet, and 1678 cm-1 for b-turn. However, if the sample is dissolved in water, the water bending at about 1640cm-1 makes the determination very difficult without removing the water. We are thinking to use NIR to work on this problem. Now we need to know which wavenumber ( or wavelength) corresponds to a-helix, b-sheet and b-turn of peptide in NIR. We need some reference. Any suggestion is welcomed.
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Tony Davies (Td)
Posted on Friday, July 15, 2005 - 5:40 am:   

Hi Heather,

I have just run a search on the JNIRS database and found the following:
NIR-99 p63
NIR-99 p9
NIR-95 p44
JNIRS 6, A253 (1998) [NIR-97]
JNIRS 3, 73 (1995)

NIR-9? are proceedings of the ICNIRS conference in 199? JNIRS is the Journal of Near Infrared Spectroscopy.
See the NIR Publications website for details.

Best wishes
Tony
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Heather Jones
Posted on Monday, August 01, 2005 - 2:50 pm:   

Thanks. Tony

Heather Jones

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