| Author | Message | ||
     Erik Skibsted |
Hi Today we had some scientific discussion about some drug products we have. Its a protein drug product where the protein is present in a solution in two ways i.e. 1) as dissolved protein and 2) as protein crystals (preticipatet with another molecule). The effectivenes of the drug is to have a certain ratio between the dissolved protein and the protein as crystal. I was wondering if anybody has experience in analyzing this type of complex mixture with NIR? At this point I am not aware of the physical apperance of a typical sample, but I have a idea that it would a slurry solution and not like a two-phase seperated mixture i.e. the crystals are not lying on the buttom in the sample vial. Could the degree of crystallization meaby be determined indirectly using some kind of scatter correction equation i.e. determine how much correction a spectrum needed to fit a reference spectrum (i.e. something from the MSC equation)?? Could that meaby be used to quantitative determination of the crystalized part? And could a simpel PLS model be fitted to the dissolved protein content?? What about OSC? and wavelet preprocessing, meaby some parameters could be estimated from the individual spectra and correlated to the degree of crystallization?? The solution contains: dissolved protein protein crystals water phenol glycerol potasium phosphat (Zn) (detergent) I would be happy to get a few references if possible! An a fast sidequestion, but not NIR related. I meet a person who told about aquistics for measuring crystallization/fibril formation because it changed the solution density - any comments?? Looking forward to hear your comments. And thankyou for a great resource in my NIR work. | ||
| yonll |
Hello, I am quite interested in your problems of complex mixture systems. To be credited, I have depth knowledge of NIR characterization of self-associated and hydrogen bonded model molecules, and have subsequently succeeded in applying such a knowledge to explore NIR features of biological matrixes for a variety of proposes. I have considerable NIR related publications in Applied Spectroscopy and J. NIR spectroscopy. I could be reached by the e-mail at [email protected]. |