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ZHENQI SHI (shizhenq)
New member
Username: shizhenq

Post Number: 1
Registered: 3-2010
Posted on Wednesday, April 20, 2011 - 10:58 pm:   

Hi,

There have been quite a bit publications on using NIR for cell culture medium. One of the review paper is as follows: Biotechnology Advances 27 (2009),726-732

Regarding the commerical system, I recalled Robert Mattes from FOSS presented a talk in EAS a few years ago about using one of their systems on some collaboration work on bio-reactor related stuffs.

Good luck!

Zhenqi (Pete) Shi
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Howard Mark (hlmark)
Senior Member
Username: hlmark

Post Number: 414
Registered: 9-2001
Posted on Wednesday, April 20, 2011 - 1:58 pm:   

Fernando - I assume that by "IR" you mean the mid-IR spectral region (wavelengths longer than 2.5 micrometers / 4000 wavenumbers). In that case, water is indeed a very strong absorber in that region, much stronger than in the Near-IR region. Ordinary transmission spectroscopy of an aqueous solution in that region is almost impossible except for a few fairly narrow spectral "windows".

As you said, ATR can be used and you can thereby obtain mid-IR spectra in the aqueous solutions, since the effective pathlength is correspondingly small. However, due to those small pathlengths, concentrations of the solutes have to be fairly high, in order for them to absorb enough to be measurable, or even detectable.

Howard

\o/
/_\
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wenli zhou (wendyz)
New member
Username: wendyz

Post Number: 2
Registered: 4-2011
Posted on Wednesday, April 20, 2011 - 9:32 am:   

Thank you all!
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Jerry Jin (jcg2000)
Senior Member
Username: jcg2000

Post Number: 41
Registered: 1-2009
Posted on Tuesday, April 19, 2011 - 4:54 pm:   

Here is one commercial NIR used for metabolomic profiling.

http://www.molecularbiometrics.com/

Jerry Jin
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Fernando Morgado (fmorgado)
Advanced Member
Username: fmorgado

Post Number: 22
Registered: 12-2005
Posted on Tuesday, April 19, 2011 - 3:12 pm:   

Hello :
Cell culture media have very high water amount, I think probably in NIR you can not see changes since water bands overlap any other absortion. Maybe IR is better than NIR, in IR water bands are not strong and you can use ATR ( very little amount of sample is necesary)
But, only is my opinion
Regards
Fernando
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wenli zhou (wendyz)
New member
Username: wendyz

Post Number: 1
Registered: 4-2011
Posted on Tuesday, April 19, 2011 - 3:06 pm:   

I have two questions here and would like to get your help:
1. Is NIR an appropriate tool to profile the composition change of cell culture media before and after culturing?
2. If so, does anyone know any NIR instrument that can be used to profile cell culture media in small quantity such as 10 to 30 ul?
Thank you very much!
Wendy

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