Abstract

European Journal of Mass Spectrometry
Volume 16 Issue 4, Pages 531–538 (2010)
doi: 10.1255/ejms.1074

Structural characterization of lipid A in food borne pathogen Cronobacter sakazakii

Chan Zhang, Yanyan Li, Guanjun Tao, Ye Li and Xiaoyuan Wang^*
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. E-mail: xiaoyuanwang@hotmail.com

We have characterized the structure of lipid A in food borne pathogen Cronobacter sakazakii BAA-894. Lipid A molecules were purified by mild acid hydrolysis, DEAE-cellulose anion exchange chromatography and preparative thin-layer chromatography. Its structure was analyzed by electrospray ionization/mass spectrometry. Two lipid A species have been found in C. sakazakii BAA-894. The majority has a structure of disaccharide of glucosamine, phosphorylated at 1- and 4′-positions, and acylated with primary 3- hydroxymyristoyl chains at 2-, 3-, 2′- and 3′-positions and secondary myristoyl residues at 2′- and 3′-positions. The other contains a secondary lauroyl chain at 2′-position in place of the myristoyl residue.

Keywords: lipopolysaccharide, lipid A, Cronobacter sakazakii, ESI/MS/MS

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