Abstract

European Journal of Mass Spectrometry
Volume 15 Issue 2, Pages 361–365 (2009)
doi: 10.1255/ejms.942

Letter: Collision energy and cone voltage optimisation for glycopeptide analysis

Judit Krenyacz, László Drahos and Károly Vékey
Chemical Research Center, Hungarian Academy of Sciences, H-1025 Budapest, Pusztaszeri út 59-67, Hungary

Instrument tuning commonly used for peptide analysis and for proteomics causes a high degree of fragmentation for glycopeptides. This results in a strongly biased glycosylation pattern. To obtain correct results for glycopeptides, both the cone voltage and the collision energy has to be reduced significantly. A suitable standard for tuning the instrument for glycopeptide analysis is aspartic acid (which fragments under similar conditions as glycopeptides); while low mass sugar fragments (for example, at 657.3 Da) are good indicators for the presence/absence of glycopeptide fragmentation.

Keywords: N-glycosylation, glycopeptide, glycoprotein, mass spectrometry, MS/MS, energy dependence, fragmentation, proteomics, glycomics

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