Abstract

European Journal of Mass Spectrometry
Volume 14 Issue 6, Pages 419–422 (2008)
doi: 10.1255/ejms.964

Letter: Analysis of complex oligosaccharides using graphitized carbon liquid chromatography/mass spectrometry

Lívia Budai,^a Ferenc Pollreisz,^a Olivér Ozohanics,^a Krisztina Ludányi,^b László Drahos^a and Károly Vékey^a,*
aHungarian Academy of Sciences, Chemical Research Center, H-1025, Pusztaszeri 59–67, Budapest, Hungary. E-mail: vekey@chemres.hu
^bSemmelweis University, Department of Pharmaceutics, H-1092. Hőgyes Endre 7, Budapest, Hungary

The sugar fraction of α-1 acid glycoprotein (AGP) was studied using porous graphitized carbon (PGC) chromatography coupled to mass spectrometry. Electrospray ionization provides efficient control over fragmentation; at low collision energy only molecular species were observed, allowing accurate oligosaccharide profiling. PGC chromatography was useful separating 18 sugars differing in monosaccharide composition. Most of these were separated into several isomeric forms; altogether 49 different oligosaccharides were found in AGP.

Keywords: oligosaccharide, glycoprotein, glycoform, isoform, HPLC, UPLC, mass spectrometry, graphitized carbon chromatography (GPC)

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