Abstract

European Journal of Mass Spectrometry
Volume 14 Issue 6, Pages 411–417 (2008)
doi: 10.1255/ejms.978

Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples

Václav Ranc,^a Vladimír Havlíček,^a,b Petr Bednar^a and Karel Lemr^a,b,*
aDepartment of Analytical Chemistry, Palacky University, Svobody 8, 77146, Olomouc, Czech Republic. E-mail: Karel.Lemr@upol.cz
^bAcademy of Sciences of the Czech Republic, Institute of Microbiology, Videnska 1083, 142 00 Prague 4, Czech Republic

A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all analytes, L- phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 µL samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment. Irrespective of a laborious initial nano-DESI set-up, the combination of the kinetic method with nano-desorption electrospray has, for the first time, been demonstrated as a promising tool for chiral analysis of drugs in blood samples.

Keywords: nano-desorption electrospray, mass spectrometry, kinetic method, chiral analysis, drug analysis, human blood samples

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