Abstract

European Journal of Mass Spectrometry
Volume 13 Issue 2, Pages 105–113 (2007)
doi: 10.1255/ejms.868

Account: Chemical cross-linking and mass spectrometry as structure determination tools

Petr Novak^a,* and Anastassios E. Giannakopulos^b,*
aInstitute of Microbiology, Academy of Sciences, Prague, Czech Republic
^bDepartment of Chemistry, University of Warwick, Coventry CV4 7AL, UK

Chemical cross-linking is becoming a valuable tool for the high-order structure determination of proteins and protein complexes. Cross-linking methodology is able to provide low-resolution structures when at least something is known already about the proteins under investigation. The suitability of top-down and bottom-up methodologies is discussed and further potential applications of chemical cross-linking of proteins, as well as combinations with other techniques such as hydrogen/deuterium exchange and molecular modeling, are suggested.

Keywords: cross-linking, protein, high order structure, mass spectrometry, top-down, bottom-up, FT-ICR

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