Abstract

European Journal of Mass Spectrometry
Volume 10 Issue 6, Pages 755–758 (2004)
doi: 10.1255/ejms.697

Isolation of isomers based on hydrogen/deuterium exchange in the gas phase

Ulf Mazurek,^a,* Melinda A. McFarland,^b Alan G. Marshall,^b,c Chava Lifshitz^a*
aDepartment of Physical Chemistry and The Farkas Center for Light Induced Processes, The Hebrew University of Jerusalem, Giv’at Ram, Jerusalem 91904, Israel. E-mail: ykv@chem.ch.huji.ac.il
^bDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA
^cIon Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, FL 32310-4005, USA

A method to isolate isomers in a Fourier transform ion cyclotron resonance mass spectrometer on the basis of their different hydrogen/deuterium exchange rates has been developed and applied to the protonated serine octamer cluster. Isolation allows interrogation of each isomer by infrared multi-photon dissociation to determine any differences in the building blocks that form these clusters. The experimental results strongly support previous assignments of an all-zwitterion structure to the slower-exchanging isomer and an all-neutral structure to its faster-exchanging counterpart.

Keywords: mass spectrometry, ion cyclotron resonance, ICR, FT-ICR, FT-MS, infrared multi-photon dissociation, IRMPD, gas-phase ­chemistry, hydrogen/deuterium exchange, isomer isolation, serine octamer, amino acid clusters

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