|
Letter: Specific isotope labeling for the identification of free N-terminal peptide of
proteins separated by polyacrylamide gel electrophoresis Aniel Sanchez, Yassel Ramos, Yanni Solano, Luis Javier González, Lazaro Betancourt, Jeovanis
Gil, Gabriel Padron and Vladimir Besada*
Mass Spectrometry Laboratory, Department of Proteomics, Center for Genetic Engineering and Biotechnology,
Havana, Cuba. E-mail: vladimir.besada@cigb.edu.cu
ABSTRACT:
We report here a method for the identification of free N-terminal peptide of in gel digested isolated proteins. It is based
in the difference between the isotopic ion distribution of N-terminal peptide and internal peptides. After guanidination of lysine residues, the primary amino groups of the gel-
entrapped protein are blocked with an equimolar mixture of normal and deuterated acetic anhydride. Upon MS analysis internal peptides display a normal isotopic ion distribution
while the N-terminal peptide shows a complex isotopic ion distribution.
Keywords:
N-terminal peptide, proteins, identification
Back to Table of Contents |
RSS Feed
