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High detection sensitivity achieved with cryogenic detectors in combination with
matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry P. Christ,* S. Rutzinger, W. Seidel, S. Uchaikin and F.
Pröbst
Max Planck Institut für Physik, Föhringer Ring 6, 80805 München, Germany. E-mail: christ@mppmu.mpg.de
C. Koy and
M.O. Glocker
Proteome Center Rostock, Joachim-Jungius-Str. 9, 18059 Rostock, Germany
ABSTRACT:
Cryogenic detectors directly measure the impact energy of any impinging
particle independent of its velocity. Thus a very high, mass-independent, detection efficiency is expected from their application in TOF-MS. The cryogenic detector applied here is
based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series using standard
sample preparation procedures shows that the detection limit for insulin (Mr: 5,734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target.
Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range,
respectively, were established.
Keywords:
cryogenic detector, time-of-flight mass spectrometry, MALDI, insulin, detection limit
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